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situ hybridization kit plus  (Agilent technologies)


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    Structured Review

    Agilent technologies situ hybridization kit plus
    Situ Hybridization Kit Plus, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/situ+hybridization+kit+plus/us11725050-2535-17-16
    Average 90 stars, based on 1 article reviews
    situ hybridization kit plus - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Transcriptome analysis reveals unique metabolic features in the Cryptosporidium parvum Oocysts associated with environmental survival and stresses
    Article Snippet: The quality of each cRNA probes was verified by total yield and specificity calculated based on NanoDrop ND-1000 spectrophotometer measurement (NanoDrop Technologies) (Wilmington, DE). .. Only probes with specificity >8 were used for hybridization using an In Situ Hybridization Kit Plus (Agilent Technologies). .. Each array was incubated with an equal amount of Cy3 and Cy5 probes (300 ng) derived from a pair of control and treated samples at 65°C for 17 h in Agilent's microarray hybridization chambers.

    Article Title: eRNAs and Superenhancer lncRNAs Are Functional in Human Prostate Cancer
    Article Snippet: .. The hybridization solution was prepared according to the in situ hybridization kit plus (Agilent Technologies, Santa Clara, CA, US). .. Hybridization was carried out using a custom microarray at 60°C for 18 h. After hybridization, the slide was scanned on an Agilent Microarray Scanner (Agilent Technologies, Santa Clara, CA, US).

    Article Title: Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress
    Article Snippet: .. Hybridization solution was prepared with 825 ng each of Cy3- and Cy5-labelled cRNA preparations using an in situ Hybridization Kit Plus (Agilent Technologies). ..

    Article Title: New Microbicidal Functions of Tracheal Glands: Defective Anti-Infectious Response to Pseudomonas aeruginosa in Cystic Fibrosis
    Article Snippet: Briefly, 300 ng of total RNA and cyanine 3-labeled CTP (Cy-3) fluorescent dyes were used to generate fluorescent cRNA with Low RNA Input Fluorescent Amplification Kit (Agilent Technologies). .. Hybridization was performed for 17 h at 65°C using the In situ Hybridization Kit Plus (Agilent Technologies). ..

    In Situ Hybridization:

    Article Title: Transcriptome analysis reveals unique metabolic features in the Cryptosporidium parvum Oocysts associated with environmental survival and stresses
    Article Snippet: The quality of each cRNA probes was verified by total yield and specificity calculated based on NanoDrop ND-1000 spectrophotometer measurement (NanoDrop Technologies) (Wilmington, DE). .. Only probes with specificity >8 were used for hybridization using an In Situ Hybridization Kit Plus (Agilent Technologies). .. Each array was incubated with an equal amount of Cy3 and Cy5 probes (300 ng) derived from a pair of control and treated samples at 65°C for 17 h in Agilent's microarray hybridization chambers.

    Article Title: Identification and developmental expression of the full complement of Cytochrome P450 genes in Zebrafish
    Article Snippet: The Agilent Low-Input QuickAmp Labeling Kit was used for labeling, the samples were hybridized to a the custom Agilent 4 × 44K feature zebrafish microarray using the Agilent In situ Hybridization Kit Plus, and labeled cDNA was combined with the Agilent 10× Control Targets (to identify microarray corners). .. Post-hybridization, microarray slides were washed as per the Agilent In situ Hybridization Kit Plus. .. Arrays were scanned with an Agilent DNA Microarray Scanner.

    Article Title: eRNAs and Superenhancer lncRNAs Are Functional in Human Prostate Cancer
    Article Snippet: .. The hybridization solution was prepared according to the in situ hybridization kit plus (Agilent Technologies, Santa Clara, CA, US). .. Hybridization was carried out using a custom microarray at 60°C for 18 h. After hybridization, the slide was scanned on an Agilent Microarray Scanner (Agilent Technologies, Santa Clara, CA, US).

    Article Title: Antimetastatic gene expression profiles mediated by retinoic acid receptor beta 2 in MDA-MB-435 breast cancer cells
    Article Snippet: Labelled RNA was further purified using the Qiagen RNeasy Mini kit protocol for liquid samples (Valencia, CA). .. Four pairs of labelled cRNA were prepared from two independent RNA extractions, hybridized to the Agilent Human 1A Oligo Array, and washed using the Agilent In situ Hybridization Kit Plus. cRNA was labelled with Cy5 and Cy3 for "swapped"-labelling for co-hybridizations on different chips. ..

    Article Title: Anti-interleukin-33 antibodies and uses thereof
    Article Snippet: Microarray Analysis Total RNA was converted to double-stranded cDNA and then to CY® dye-labeled cRNA using an Agilent Fluorescent Linear Amplification kit. .. CY® dye-labeled cRNA was fragmented and hybridized to Agilent's whole mouse genome array as described in Agilent's In Situ Hybridization Kit Plus. ..

    Article Title: Comparative analysis of root transcriptome profiles of two pairs of drought-tolerant and susceptible rice near-isogenic lines under different drought stress
    Article Snippet: .. Hybridization solution was prepared with 825 ng each of Cy3- and Cy5-labelled cRNA preparations using an in situ Hybridization Kit Plus (Agilent Technologies). ..

    Article Title: New Microbicidal Functions of Tracheal Glands: Defective Anti-Infectious Response to Pseudomonas aeruginosa in Cystic Fibrosis
    Article Snippet: Briefly, 300 ng of total RNA and cyanine 3-labeled CTP (Cy-3) fluorescent dyes were used to generate fluorescent cRNA with Low RNA Input Fluorescent Amplification Kit (Agilent Technologies). .. Hybridization was performed for 17 h at 65°C using the In situ Hybridization Kit Plus (Agilent Technologies). ..

    Article Title: IL-16 Promotes T. whipplei Replication by Inhibiting Phagosome Conversion and Modulating Macrophage Activation
    Article Snippet: Briefly, labeled cDNA was synthesized using the Low RNA Input Fluorescent Amplification Kit (Agilent Technologies) with 300 ng of total RNA and cyanine 3-labeled CTP. .. Hybridizations of 4X44k Mouse Whole Genome microarrays (Agilent Technologies) were performed in triplicate for 17 hours at 65°C using the In situ Hybridization Kit Plus (Agilent Technologies). .. Slides were scanned at a 5-μm resolution by a G2505B DNA microarray scanner (Agilent Technologies), and images were analyzed with the Agilent Feature Extractor Software 9.5.1.1.

    Microarray:

    Article Title: Identification and developmental expression of the full complement of Cytochrome P450 genes in Zebrafish
    Article Snippet: The Agilent Low-Input QuickAmp Labeling Kit was used for labeling, the samples were hybridized to a the custom Agilent 4 × 44K feature zebrafish microarray using the Agilent In situ Hybridization Kit Plus, and labeled cDNA was combined with the Agilent 10× Control Targets (to identify microarray corners). .. Post-hybridization, microarray slides were washed as per the Agilent In situ Hybridization Kit Plus. .. Arrays were scanned with an Agilent DNA Microarray Scanner.



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    (A) Simulated solar irradiation (SSR) of abdominal areas, at doses of 2 and 4 J/cm 2 , five hours before plastic surgery. Five women with a median age of 42 years, with phototype of II or III according to Fitzpatrick classification, were included in a photobiological study. (B) <t>Microarray</t> workflow. Non-irradiated and irradiated samples were separately compared to a reference, containing pooled samples (non-irradiated and irradiated). Significance Analysis of Microarrays (SAM) and a false discovery rate (FDR) of zero were used to identify transcripts that were differentially expressed between irradiated and non-irradiated conditions. SAM comparing 2 J/cm 2 with non-irradiated samples identified 288 differentially regulated genes; comparison between 4 J/cm 2 -irradiated and non-irradiated samples identified a set of 473 differentially regulated genes; and 3-class SAM identified a set of 476 genes .
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    Agilent technologies hybridization buffer situ hybridization plus kit
    (A) Simulated solar irradiation (SSR) of abdominal areas, at doses of 2 and 4 J/cm 2 , five hours before plastic surgery. Five women with a median age of 42 years, with phototype of II or III according to Fitzpatrick classification, were included in a photobiological study. (B) <t>Microarray</t> workflow. Non-irradiated and irradiated samples were separately compared to a reference, containing pooled samples (non-irradiated and irradiated). Significance Analysis of Microarrays (SAM) and a false discovery rate (FDR) of zero were used to identify transcripts that were differentially expressed between irradiated and non-irradiated conditions. SAM comparing 2 J/cm 2 with non-irradiated samples identified 288 differentially regulated genes; comparison between 4 J/cm 2 -irradiated and non-irradiated samples identified a set of 473 differentially regulated genes; and 3-class SAM identified a set of 476 genes .
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    Image Search Results


    (A) Simulated solar irradiation (SSR) of abdominal areas, at doses of 2 and 4 J/cm 2 , five hours before plastic surgery. Five women with a median age of 42 years, with phototype of II or III according to Fitzpatrick classification, were included in a photobiological study. (B) Microarray workflow. Non-irradiated and irradiated samples were separately compared to a reference, containing pooled samples (non-irradiated and irradiated). Significance Analysis of Microarrays (SAM) and a false discovery rate (FDR) of zero were used to identify transcripts that were differentially expressed between irradiated and non-irradiated conditions. SAM comparing 2 J/cm 2 with non-irradiated samples identified 288 differentially regulated genes; comparison between 4 J/cm 2 -irradiated and non-irradiated samples identified a set of 473 differentially regulated genes; and 3-class SAM identified a set of 476 genes .

    Journal: PLoS ONE

    Article Title: In Vivo Identification of Solar Radiation-Responsive Gene Network: Role of the p38 Stress-Dependent Kinase

    doi: 10.1371/journal.pone.0010776

    Figure Lengend Snippet: (A) Simulated solar irradiation (SSR) of abdominal areas, at doses of 2 and 4 J/cm 2 , five hours before plastic surgery. Five women with a median age of 42 years, with phototype of II or III according to Fitzpatrick classification, were included in a photobiological study. (B) Microarray workflow. Non-irradiated and irradiated samples were separately compared to a reference, containing pooled samples (non-irradiated and irradiated). Significance Analysis of Microarrays (SAM) and a false discovery rate (FDR) of zero were used to identify transcripts that were differentially expressed between irradiated and non-irradiated conditions. SAM comparing 2 J/cm 2 with non-irradiated samples identified 288 differentially regulated genes; comparison between 4 J/cm 2 -irradiated and non-irradiated samples identified a set of 473 differentially regulated genes; and 3-class SAM identified a set of 476 genes .

    Article Snippet: Hybridization was performed using an Agilent oligonucleotide microarray in situ Hybridization-Plus kit, following the manufacturer's instructions.

    Techniques: Irradiation, Microarray

    Genes showing differential regulation only in response to the higher dose of SSR are annotated with several GO terms. Up-regulated genes (A) were associated with terms that included negative regulation of cellular processes, morphogenesis of anatomical structures, JAK-STAT signaling, nucleosome assembly and the immune response (inflammatory response and cell differentiation were also found for 1 MED (minimal erythema dose: 2 J/cm 2 ), as indicated in red); for down-regulated genes (B), GO terms were predominantly associated with transcription and regulation, although three GO terms (in red) relating to “regulation of transcription” were also observed for 1 MED . (C) Microarray data were validated for 11 genes (nine up and two down-regulated): mRNA was assayed by qPCR and normalized to the values for 18S mRNA. These genes were selected from the list of differentially expressed genes, for their classification as inflammatory response genes or their involvement in the p53 pathway. Error bars represent standard deviation. Stars indicate significant differences (two-tailed Student's t-test) between control and irradiated samples: * P<0.05; ** P<0.01; *** P<0.001.

    Journal: PLoS ONE

    Article Title: In Vivo Identification of Solar Radiation-Responsive Gene Network: Role of the p38 Stress-Dependent Kinase

    doi: 10.1371/journal.pone.0010776

    Figure Lengend Snippet: Genes showing differential regulation only in response to the higher dose of SSR are annotated with several GO terms. Up-regulated genes (A) were associated with terms that included negative regulation of cellular processes, morphogenesis of anatomical structures, JAK-STAT signaling, nucleosome assembly and the immune response (inflammatory response and cell differentiation were also found for 1 MED (minimal erythema dose: 2 J/cm 2 ), as indicated in red); for down-regulated genes (B), GO terms were predominantly associated with transcription and regulation, although three GO terms (in red) relating to “regulation of transcription” were also observed for 1 MED . (C) Microarray data were validated for 11 genes (nine up and two down-regulated): mRNA was assayed by qPCR and normalized to the values for 18S mRNA. These genes were selected from the list of differentially expressed genes, for their classification as inflammatory response genes or their involvement in the p53 pathway. Error bars represent standard deviation. Stars indicate significant differences (two-tailed Student's t-test) between control and irradiated samples: * P<0.05; ** P<0.01; *** P<0.001.

    Article Snippet: Hybridization was performed using an Agilent oligonucleotide microarray in situ Hybridization-Plus kit, following the manufacturer's instructions.

    Techniques: Cell Differentiation, Microarray, Standard Deviation, Two Tailed Test, Irradiation